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No. Researcher Title Abstract Year
1 San San Aye COMPARATIVE STUDIES OF ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL CONSTITUENTS OF SOME MYANMAR INDIGENOUS MEDICINAL PLANTS Developing countries, where dysentery and diarrhea are endemic, depend strongly on traditional medicine as a source for inexpensive treatments because it is based on plants which are abundantly available in these countries. Consequently, Myanmar indigenous medicinal plants (Curcuma longa L., Nigella satia L. and Piper betel L.) which are used for the treatment of dysentery and diarrhea in Myanmar were selected to study in order to find the scientific basis for such use. The selected plants were screened for antibacterial activity by using agar disc diffusion technique. Polar and non-polar extracts of the selected plants were tested on 33 species of standard and hospital bacterial strains. The Minimum Inhibitory Concentration (MIC) of the active extracts was also determined by agar plate dilution method. Three curcuminoids, namely curcumin (5.9 %), desmethoxy curcumin (0.018 %) and bisdesmethoxy curcumin (0.0136 %) were isolated from C. longa rhizomes. N. satia seeds were fractionated by column chromatography to give thymoquinone (0.01 %), kaempferol (0.12 %) and quercetin (0.001 %). Eugenol (0.1 %) was isolated from essential oil of P. betel. The isolated compounds were identified by UV, FT IR, 1H NMR, 13C NMR and mass spectroscopic methods. The isolated compounds were also found to show bactericidal activity. Thus the extracts of the three plants have high potential for the production of combined formulation to treat infections caused by bacteria 2018
2 MyoMin STUD ON PHTOCONSTITUENTS AND BIOACTIVE PROPERTIES OF AVOCADO (rsa Amricana MILL.) SEEDS Avocado (Persea americana Mill.) is well known traditional medicinal plant and one of the most important fruit crops of the local market in Myanmar. According to the phytochemical investigation of avocado seeds, it was observed that phenolic compounds and flavonoids were present in the fruit seeds. Semi-quantitative elemental analysis of avocado seeds was performed by EDXRF method. Potassium and calcium were present as the considerable amounts in the avocado seeds. The nutritional values of avocado seeds were determined by AOAC method. It was observed that carbohydrate was the highest content and protein, fat and fiber were low contents in the sample. Screening of antimicrobial activities from avocado seeds was carried out by agar well diffusion method. In the screening, different crude extracts such as pet-ether, chloroform, ethylacetate, ethanol, methanol and water of avocado seeds were examined with the six microorganisms. According to the antimicrobial screening, avocado seeds possess antimicrobial activity. The antioxidant activity of water and ethanolic extract from avocado seeds was determined by DPPH free radical scavenging activity method using UV spectrophotometer. The ascorbic acid was used as a standard. The IC50 values of standard ascorbic acid, water and ethanolic extract were observed as 37.05, 45.05 and 93.17 µg / mL, respectively. Therefore, the antioxidant activity of water extract from avocado seed was more potent than that of ethanolic extract 2018
3 MinMinYee FABRICATION OF BIMETAL OXIDE HOLLOW NANOSPHERE USING CORE-CORONA MICELLES AS SOFT TEMPLATE Currently, nanoparticles with controlled morphology have tremendously attracted attention due to their wide application in catalysis, energy storage, and biomedicine. It is well known that hollow nanospheres with controlled size and shape show better performance compared to the conventional dense nanoparticles. In this research, Co/Fe oxide hollow nanospheres with diameter around 27?2 nm have been fabricated by using polymeric micelles of poly (styrene-block-acrylic acid) (PS-b-PAA) as a template. The polymer forms spherical micelles with PS-core and PAA-corona in aqueous solutions. According to the transmission electron microscopic (TEM) result, the void space of the fabricated hollow nanospheres is around 17 ?1 nm. The prepared Co/Fe oxide hollow nanospheres became shrunk during calcination. Fourier transform infrared resonance (FTIR) measurements confirm that the template polymer was completely removed during calcination 2018
4 Thandar Aung SCREENING OF THE ACUTE TOXICITY ANTIMICROBIAL AND ANTITUMOR ACTIVITIES OF ROOTS OF Stemona Curtisii HOOK.F. MYANMAR INDIGENOUS MEDICINAL PLANTS In the present research, roots of Stemona curtisii Hook. F., family- Stemonaceae was chosen to be studied. Acute toxicity study of 95% ethanol extract from roots of Stemona curtisii Hook. F. was investigated by methods of OECD guidelines for the testing of Chemical 425. Screening of root extract was done with the dosage of 2000 mg/kg, 300 mg/kg and 50 mg/kg body weight in albino mice. Dosage of 2000 mg/kg was discovered lethality within 60 min with symptoms of toxicity like restlessness, convulsion, coma, and death. The results of other groups show no lethality of mice up to fourteen days administration. Antimicrobial activity of pet- ether, methanol, ethyl acetate, 95% ethanol and watery extracts from roots of S .curtisii was investigated against six species of microorganisms such as Bacillus pumilus, Bacillus subtilis, Candida albicans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus by agar well diffusion method. Ethyl acetate and methanol extracts of S. curtisii exhibited inhibition zone diameter in the ranges of (12 15 mm) and (11 20 mm) against all tested microorganisms, respectively. 95% ethanol extract of S.curtisii showed inhibition zone diameters (11 mm) only against Pseudomonas aeruginosa. On the other hand, pet-ether and watery extract of sample showed activity against five tested microorganisms in the range of (11 14 mm) and (11 20 mm) except Bacillus subtilis. Antitumor activity of methanol, ethyl acetate, 95% ethanol, and watery extracts of roots of S.curtisii was screened on Agrobacterium tumefacien by Potato Disc Assay method. All of extracts from root sample exhibited antitumor activity against Agrobacterium tumefacien after 5 days and 7 days periods of observation 2018
5 Sandar Moe STUD ON PHTOCONSTITUENTS AND BIOACTIVE PROPERTIES OF AVOCADO (rsa Amricana MILL.) SEEDS The Smallanthus sonchifolius (yacon) tubers were dried at the ambient temperature, chopped into bits and percolated with70% ethanol. Qualitative phytochemical screening was determined by the standard procedure suggested the presence of alkaloids, flavonoids, steroids, polyphenols, terpenoids,-amino acids, glycosides, phenolic compounds, carbohydrate, saponins, tannins, protein, and reducing sugars. Starch and cyanogenic glycosides were not detected in yacon tubers. The profile of the chemical constituents present was established by thin layer chromatography. Six pure organic constituents namely, stigmasteryl acetate (0.003%), ?-sitosteryl acetate (0.005%), hexadecanoic acid (0.009%), ?-sitosterol (0.02%), chlorogenic acid (0.12%) and ?-sitosterol ?-D-glucoside (0.8%) based on ethyl acetate extract were isolated from the ethyl acetate soluble fraction of the 70% ethanol extract of the yacon tuber on a silica gel column by gradient elution with petroleum and ethyl acetate (PE-EA, 99:1 to EA only). The isolated compounds were characterized by UV and FTIR. 2018
6 Arnt Win STUDY ON CONSTITUENTS IN MYANMAR TRADITIONAL MEDICINAL PLANT oloptelea integriolia (ROXB.) PLANCH (Roxb.) Planch (Ulmaceae) is a tree widely distributed on the Myanmar, India, Indochina and Asia, and it is locally called in Myanmar as “Pyauk-seik”. The various extracts of stem bark of were analyzed by Thin Layer and Column Chromatography method. The stem barks of were collected from Chaik Village, Pakokku Township, Magway Region, Myanmar. They were washed, driedin shade, weighed and cut into small pieces and extracted in methanol. The fractionation of crude extract, followed by the addition of distilled water, n-hexane , ethyl acetate and n-butanol to an aqueous portion of each solvent, to obtain the dried masses of each four layers. Three pure compounds such as new 2-(2-hydroxyethylamino)-1,4 -naphthoquinone (1),(-)-syringaresinol (2), and(+) (4S)-4-hydroxy--tetralone (3) are isolated from ethyl acetate portion of this plant. The structures of these compounds were elucidated by analysis of NMR spectroscopic and mass spectrometric data. Although compound (1) is synthetically known, to the best of our knowledge, there is no previous report of its natural occurrence. The compounds (2 and 3) are described for first time from species. Moreover, the antioxidant activity of compound3was evaluated by measuring the half inhibition concentration (IC50) using chemiluminescent method. This isolated compound was found to exhibit significant antioxidant property which is comparable to standard ascorbic acid, at a specific concentration. 2018
7 Khin Mar Cho BIOSYNTHESIS OF INC OXIDE NANOPARTICLESUSING CAPS ANAL. (MA-YO-GYI)CHARACTERIATION AND ANTIMICROBIAL ACTIVITIES This research deals with biosynthesis of zinc oxide nanoparticles using Calotropis giganteaL. (ma-yo-gyi), characterization and antimicrobial activities. The aim of this research is to synthesize, characterize and study the antimicrobial activities of biosynthesized zinc oxide nanoparticles. Leaves of Calotropis gigantea L. (Ma-yo-gyi) were collected from Kathitkan village in Aung Lan Township, Magway Region and identified in Department of Botany, Pyay University. The phytochemical screening of aqueous leaves extract of Calotropis gigantea L. was carried out by test tube method and aqueous leaves extract was also characterized by FT IR technique. Zinc oxide nanoparticles were biosynthesized fromzinc nitrate by method Iand method II using aqueous leaves extract of Calotropis gigantea L. as a reducing agent. Thermal stability of green synthesized zinc oxide nanoparticles by method I was studied by Thermogravimetric-Differential Thermal Analyzer (TG-DTA). The effects of calcination temperature on crystallize sizes of biosynthesized ZnO nanoparticles by method I was also studied. The crystallinity and purity of biosynthesized zinc oxide nanoparticles by methods I and II werestudied by X-ray Diffraction (XRD) technique. The biosynthesized ZnO nanoparticles by both methods were found to be crystalline in nature and which has hexagonal structure. The average crystallize sizes were calculated by using Debye-Scherrer equation and found to be in the range of (25-33) nm. The antimicrobial activities of biosynthesized ZnO nanoparticles on six microorganisms such as B. subtilis, B. pumilus, C. albicans, S. aureus, E. coli and P. aeruginosa were studied by ager well diffusion method. It was found that the high antimicrobial activities of biosynthesized ZnO NPs (23-26 mm) on gram positive and negative bacteria strain except P. Aeruginosa and also high activity on fungus like Candida albicans. For the investigation of the storage time of guava and tomato fruits at room temperature, biosynthesized ZnO nanoparticles coated on surface of these fruits and found to be fresh without any moltuntilone month 2018
8 Thinn Myat Nwe MOLECULAR FORMULA DETERMINATION AND STRUCTURE ELUCIDATION OF BIOACTIVE PORPHYRIN DERIVATIVE COMPOUND ISOLATED FROM THE WHOLE PLANT OF Corallodiscs langinoss (WALL. ex R.Br.) BURTT In this research paper, Corallodiscus lanuginosus (Wall. ex R.Br.) Burtt which is one of the Myanmar indigenous medicinal plants known as Pan ma o was selected for chemical analysis. The preliminary phytochemical screening of the whole plant of Pan ma o was carried out, which indicated the presence of alkaloid, steroid, terpenoid, glycoside, lipophilic, polyphenol, saponin, phenolic and tannin, respectively. Antimicrobial activities of the crude extract of the whole plant of Pan ma o were tested in various solvents system by using agar well diffusion method on six selected organisms. The ethyl acetate crude extract of Pan ma o gives rise to high activities on all selected organisms such as Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and E.coli. Moreover, porphyrin derivative compound was isolated from the whole plant of Pan ma o as indigo crystal (19.6 mg) by thin layer and column chromatography separation methods. The yield percent was found to be (0.529%) based upon the EtOAc crude extract. Moreover, antimicrobial activities of this compound were rechecked by using agar well diffusion method. The ethyl acetate extract of this compound responds high activities on all selected organisms except Staphylococcus aureus. In addition, the molecular formula of this compound could be determined as C30H35N9O4 (Hydrogen Deficiency Index = 18), applying some spectroscopic methods such as FT IR, 1H NMR (500 MHz), 13C NMR (125 MHz), DEPT and FAB-mass spectral data respectively. The complete structure of this porphyrin derivative compound could be elucidated by DF-COSY, HMC, HMBC and DEPT spectroscopic methods. The elucidated compound could be described as below. 2018
9 Myint Myin Khine INVESTIGATION ON SOME PHYTOCHEMICAL CONSTITUENTS AND BIOACTIVITIES OF SEED EXTRACTS OF Mucuna Pruriens L.(KHWE-LEYA) The research focused on the investigation of some phytochemical constituents of (L.) seed and some of its biological activities. The sample was collected from Ingapu Township, Ayeyawady Region and it was identified at Department of Botany, Hinthada University. Preliminary phytochemical tests have revealed the presence of alkaloids, carbohydrates, flavonoids, ?-amino acids, starch, organic acids, phenolic compounds, saponins, glycosides, reducing sugars, steroids and terpenoids in the sample according to test tube methods and TLC profile. The seed sample was found to contain 14.39 % of moisture, 3.43 % ash, 23.22 % of protein, 8.98 % of dietary fiber, 1.68 % of crude fat, 48.30 % of carbohydrate, and 302 kcal /100 g of energy value based on dried sample. The seed sample was found to have relatively highest content of K and P whereas minor components of S, Ca, Fe, Zn, Cu and Mn according to EDXRF analysis. The antimicrobial activities of PE, EtOAc, CHCl3, 95 % EtOH and H2O extracts from seed were screened by agar well diffusion method on six species of microorganisms, namely , , , , and . All extracts of seed sample were observed to possess antimicrobial activity. Among the tested crude extracts, EtOH extract was found to possess the most potent antimicrobial activity (inhibition zone diameter ranged between 14~16 mm). antioxidant activities of 95 % EtOH and watery extracts from seed were assessed by DPPH radical scavenging activity assay. The antioxidant activity of watery extract (IC50= 3.62 g/mL) was found to be higher than ethanol extract (IC50= 4.49 g/mL). From the results of phytochemical constituents, antioxidant and antimicrobial activities of the seed observed in the present study, the seed could be applied as the local health remedy for the local indigenous communities of our country. 2018
10 Ei Ei Khaing ISOLATION OF ASARONE FROM THE RHIZOME OF Acrus caamus L. (LIN-NE) AND ANTIBACTERIAL SCREENING OF THE CRUDE EXTRACTS AND ASARONE The rhizome of L. (Lin-ne) used in the treatment of diarrhoea and dysentery in traditional Myanmar medicinal system was chosen for present study. The aim of the study is to isolate asarone from the rhizome of L. (Lin-ne) and antibacterial screening of its crude extracts and asarone. At first, four crude extracts of this plant were prepared by using various solvents; petroleum ether, ethyl acetate, 96 % ethanol and 50 % ethanol. antibacterial activity of four crude extracts was investigated against 19 bacterial strains by using agar disc diffusion method. Among the four crude extracts, the most active ethyl acetate extract was selected for isolation of active compound by using column chromatographic method. The isolated compound, asarone (0.712 %) was identified by TLC and spectroscopic methods; UV, FT IR, GC-MS and then tested on 11 bacteria; species, A, species, ATCC, ? ? YCH 149, ? species, and O1 by agar disc diffusion method. In addition minimum inhibitory concentration (MIC) of asarone was determined by microtitre plate dilution method and the MIC value 0.06 mg/mL of asarone on tested bacteria, ? LT and ? was found. 2018