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No. Researcher Title Abstract Year
1001 Thidar Cho TAXONOMIC STUDY ON TWELVE SPECIES OF FAMILY FABACEAE FROM NAT YAY GAN MOUNTAIN AREA IN NGAPHE TOWNSHIP The present research work deals with the taxonomic study on some species of family Febaceae from Nat Yay Gan mountain area in Ngaphe Township which is located in Middle part of Myanmar. This area lies between 20º10'N Latitude and 94º30'E Longitude. The flowering plants from the floristic area were collected, preserved and identified from 2017 to 2018. Altogether 12 species which are belonging to 8 genera were included. The characteristics of the family, the morphological characters of the genera and species were presented with the colour photographs. The names of the genera were arranged in alphabetically. All the plants were recorded and described. 2020
1002 Khin Nilar Oo ISOLATION AND MORPHOLOGICAL CHARACTERIZATION OF FUNGI FROM BEIKTHANO ANCIENT CITY AND THEIR ANTIMICROBIAL ACTIVITY Soil samples were collected from four different places of Beikthano Ancient city, Taungdwingyi Township, Magway Region. Twenty fungi were isolated from these four different soil samples. Isolation of fungi were undertaken by the chemical treatment method. The soil fungi were isolated by using Low Carbon Agar medium and Malt Extract Agar medium for first culture and Potato Glucose Agar medium for pure culture. The isolated fungi were given as NLF-1 to NLF-20. The surface color of isolated fungi NLF-1 to NLF-20 were white, green, orange white, greenish yellow, yellow, pale brown, black, gray and their reverse color were pale yellow, white, cream, greenish yellow, brown, gray and pink. Antimicrobial activity of isolated fungal strains was evaluated by the agar well diffusion assay with four test organisms. These isolated fugal strains were tested by using 3 days to 10 days old culture. Nine strains showed the antimicrobial activity against Agrobacterium tumefaciens, Aspergillus paraciticus, Micrococcus luteus and Pseudomonas fluorescens. Among them, NLF-14 showed the highest antibacterial activity (32.53 mm) on A. tumefaciens followed by NLF-7 (29.40 mm) and NLF-16 (29.96 mm) respectively. And then, NLF-14 also showed the highest antifungal activity (32.43 mm) on A. paraciticus followed by NLF-9 (31.09 mm) and NLF-6 (30.73 mm). Among all the selected fungi, NLF-12 showed the best inhibition zone (34.85 mm) against M. luteus.NLF-6 showed the highest antibacterial activity (30.26 mm) on P. fluorescens. 2020
1003 Nwe Ni Tin POLLEN MORPHOLOGY, PHYTOCHEMICAL TEST AND ANTIMICROBIAL ACTIVITIES OF TEA LEAVES FOUND IN WAN SAING VILLAGE, KYAING TONG From the methanolic extract of green tea leaves of Camellia sinensis (L) Kuntze, collected from Wan - Saing, a Loi (ethics minority in Myanmar) village within the Tract of Kat- Pha, Kyaing Tong District, Eastern of Shan State. Phytochemical contents of the tea leaves were analyzed and evaluated. By these analyses, carbohydrate, glycoside, phenolic compound, ? - amino acid, saponin, tannin, flavonoid, steroid, terpenoid and reducing sugar are found to be present. Notably, starch and cyanogenic glycosides were not observed in the extract sample under study. In addition the values of the nutrients from the leaf extract were examined using AOAC (Analytical Official of Chemic Method). As a result the energy value was found to be the highest with the potential value of 280 % whereas carbohydrate content is second in line with the concentration of 51.33%. The concentric percent of the remaining nutrients such as moisture, ash, crude protein, crude fiber and crude fat were found to be 11.73%, 5.02%, 10.42%, 17.80% and 3.70% respectively. Caffeine concentration was found as the lowest in concentration (0.27%). By agar-well diffusion method, using different kinds of solvents, the antimicrobial activity of constituents from the leaf extract were studied. Their inhibitory zones were also evaluated. 2020
1004 Moe Moe Win ISOLATION, IDENTIFICATION OF BACTERIA FROM MANGROVE SOIL AND THEIR ANTIMICROBIAL ACTIVITY The soil samples were collected from mangrove area at Shwe-thaung-yan Township, Ayeyawady Region. Isolation of bacteria was done by serial dilution method and cultured on Nutrient Agar (NA) medium (Atlas, 1993). As the results, total of seven bacterial strains (M-1 to M-7) were obtained and tested by agar well diffusion method with five kinds of test organisms. Four strains showed different levels of antimicrobial activities. Especially M-7 showed the best antifungal activity on Candida albicans. Therefore, M-7 was selected and identified by colony morphology, gram staining, spore staining, microscopical characters and biochemical reactions. According to the results, M-7 was characterized as the genus Pseudomonas sp. 2020
1005 Mya Min Min Myo CULTURAL CHARACTER AND ANTIMICROBIAL ACTIVITIES OF ISOLATED FUNGI FROM SOIL SAMPLES OF BELU-GYUN, CHAUNGZON TOWNSHIP, MON STATE Soil samples from twenty different locations of Belu-Gyun, Chaung-Zon Township, Mon State were collected. These soil samples were utilized in the isolation of 37 fungal strains. They were designated as MM-1 to MM-37. Among these fungi colonies, MM-34 was found to give the highest antibacterial activity of 36.35 mm clear zone against Micrococcus luteus. So, MM-34 was preferred to conduct further investigation concerning the optimal fermentation conditions to produce antibacterial metabolite. In the fermentation experiments with MM-34, it was observed that the 84 hours of ages, 15% sizes of inoculums were suitable for highest antibacterial activity against Micrococcus luteus. The result revealed that maximum production of antimicrobial metabolite (27.27 mm clear zone) was obtained when medium was supplemented with glucose. Culture containing yeast extract showed the highest antibiotic production (27.54 mm clear zone). FM-3 gave maximum yield of antibacterial activity (30.02 mm clear zone) against Micrococcus luteus. 2020
1006 Myo Htaik Aung CULTURAL CHARACTERISTICS AND ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC FUNGI FROM LEAVES OF TWELVE MEDICINAL PLANTS A total of 18 endophytic fungi were screened and isolated into pure culture from the leaves of 12 medicinal plants collected from Pathein University and Mawlamyine University Campus and Taung wine area, Mawlamyine. The isolates were designated as MHA-1 to MHA-18. Among them MHA-10 which was isolated from the leaves of Dioscorea birmanica Prain and Burkill. (Khat-Cho) showed the highest antibacterial activity (18.90 mm clear zone) against Bacillus subtilis. Thus MHA-10 was selected for further study of optimal fermentation parameter to produce antibacterial metabolites. It was recorded that 84 hrs ages of inoculums, 30% size of inoculums were found to provide the best antibacterial activity in fermentation medium-1. Maximum antibacterial activity (18.03 mm clear zone) was found when fermentation media was supplemented with glucose. Among the wide variety of nitrogen sources tested, peptone proved to be the most suitable for antibacterial activity (18.95 mm clear zone) of selected fungus MHA-10 against Bacillus subtilis. FM-1 gave maximum antibacterial activity (20.64 mm clear zone) against Bacillus subtilis. 2020
1007 Mai Bawi Nuam Thein A PRELIMINARY STUDY ON THE ANTIMICROBIAL ACTIVITIES OF ISOLATED SOIL FUNGI FROM THA-BEIK-KYIN AREA In this present study, seven different soil samples were collected in the Tha-Beik-Kyin area of Mandalay region for the isolation of soil microorganisms. After that soil fungi were isolated from different soil samples at Biological Resource and Biotechnology Development Center (BRBDC) of Botany Department in Pathein University. Eleven fungi were isolated from these seven different soil samples by using physical treatment dilution method on low carbon medium. Then, the isolated fungi were photographed and the studies were conducted on their respective morphological characters. Following that, antimicrobial activities of the fungi were tested by the paper disc diffusion assay method. In the study of antimicrobial activities, 8 different test organisms were utilized. According to the results, three isolated strains (MAI 01, MAI 06, and MAI 07) showed activity against the test organisms. Among them, strain MAI-07 showed the highest antibacterial activity against Bacillus subtilis (25.70 mm). In the fermentation studies, 84 hr age and 15% size of inoculums were optimized for the fermentation. Carbon sources (glucose, glycerol) and nitrogen sources (yeast extract, polypeptone) showed the best activities on the fermentation. 2020
1008 Ma Hnin Thiri Lwin ISOLATION AND ANTIMICROBIAL ACTIVITY OF ACTINOMYCETES FROM CHAUNG-THA AREA AND BIOCHEMICAL CHARACTERIZATION OF SELECTED STREPTOMYCES (TR-2) A total of 13 actinomycetes were isolated from two samples sea-water and sludge, ChaungTha area, Ayeyarwady region. Among them, 8 strains were obtained from sludge sample and 5 strains from sea water. In the elevation, the isolated strains were circular, raised, convex, umbonate and flat. In the margin and textures, all strains were entire and rough. The colony size of isolated strains were large, small and moderate. The aerial mass colour of all strains was greenish blue, white and centre greenish blue, white in periphery. Their substrate colour was yellow. The antimicrobial activity of all strains were screened by agar well diffusion method on ten test organisms. Of these 13 strains, eight strains showed the antimicrobial activity. Among them, TR-2 showed the highest antifungal activity on Candida albicans. Therefore, TR-2 was selected for biochemical characterization. Positive results were found in methyl red test, citrate utilization test, casein test, mannitol salt broth test, potato plug test, nitrate reduction, catalase and urease test. But, Voges proskauer test, hydrogen sulfide test, gelatin hydrolysis test, esterase activity test, oxidase test and motility test were negative. According to the results, TR-2 was classified as the possible genus Streptomyces sp. Keywords: Sea water, sludge, streptomyces, antimicrobial activity 2020
1009 Ohnmar Htwe COMPARATIVE POLLEN MORPHOLOGY OF TEN GREWIA SPECIES Pollen morphology of 10 species belonging to the genus Grewia of subfamily Tilioideae were studied. All the specimens were collected from Mandalay and Magway Regions during 2018. Plant identification was carried out and pollen grains were studied. Pollen grains of all investigated species were monad. The aperture types of pollen grains of all species were found as colporate, except Grewia villosa Willd which is colpate. The shapes of pollen grains were found as subprolate in Grewia columaris Smith, Grewia parviflora (Burge) HandenMazzetti, and Grewia villosa Willd and the remaining seven species were prolate. The sizes of pollen grains were medium and large. Among the studied species, the smallest pollen grains was found in Grewia columaris Smith and the largest pollen grains was in Grewia laevigata Vahl.ex Juss. The taxonomic descriptions of each species were described with their inflorescences. The polar view and equatorial view of the pollen for each species were presented. Moreover, the comparative pollen morphological characters were presented in table. 2020
1010 Ohnmar Htwe TAXONOMIC STUDY ON TEN WILD MUSHROOMS FROM HOPONE AND HSIHSEING TOWNSHIPS IN SOUTHERN SHAN STATE The taxonomic studies on wild mushrooms from Hopone and Hsihseing Township, Southern Shan State have been undertaken. The study area is located between N' 20º 48' - 20º 53' and E' 97º 10' - 97º 15'. The wild mushrooms were collected from June to September, 2018. The 10 species of 8 genera belonging to 7 families and 4 order were collected, preserved, classified, identified and described. The collected species were identified as Helvella crispa (Scop.) Fr., Auricularia auricular-judage (Bull.) Wettst., Amanita caesarea (Scop.) Pers., Calvaria aurea (Fr.) Quel., Pleurotus lignatilis (Pers.) Rehdead & Ginns., Pleurotus pulmonarius (Fr.) Quel., Schizophyllum commune (Fr.) Fr., Lactarius determinus Groger., Lactarius volemus Fr., Russula cyanoxantha (Schaeff.) Fr. and Russula delica Fr. The growing habitats of Auricularia auricular-judage (Bull.) Wettst. and Schizophyllum commune (Fr.) Fr. were on the decayed woods and the others were on the soil. An artificial key to the studied species was constructed and presented. 2020