SCREENING OF THE ACUTE TOXICITY ANTIMICROBIAL AND ANTITUMOR ACTIVITIES OF ROOTS OF Stemona Curtisii HOOK.F. MYANMAR INDIGENOUS MEDICINAL PLANTS
Abstract
- In the present research, roots of Stemona curtisii Hook. F., family- Stemonaceae was chosen to be studied. Acute toxicity study of 95% ethanol extract from roots of Stemona curtisii Hook. F. was investigated by methods of OECD guidelines for the testing of Chemical 425. Screening of root extract was done with the dosage of 2000 mg/kg, 300 mg/kg and 50 mg/kg body weight in albino mice. Dosage of 2000 mg/kg was discovered lethality within 60 min with symptoms of toxicity like restlessness, convulsion, coma, and death. The results of other groups show no lethality of mice up to fourteen days administration. Antimicrobial activity of pet- ether, methanol, ethyl acetate, 95% ethanol and watery extracts from roots of S .curtisii was investigated against six species of microorganisms such as Bacillus pumilus, Bacillus subtilis, Candida albicans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus by agar well diffusion method. Ethyl acetate and methanol extracts of S. curtisii exhibited inhibition zone diameter in the ranges of (12 15 mm) and (11 20 mm) against all tested microorganisms, respectively. 95% ethanol extract of S.curtisii showed inhibition zone diameters (11 mm) only against Pseudomonas aeruginosa. On the other hand, pet-ether and watery extract of sample showed activity against five tested microorganisms in the range of (11 14 mm) and (11 20 mm) except Bacillus subtilis. Antitumor activity of methanol, ethyl acetate, 95% ethanol, and watery extracts of roots of S.curtisii was screened on Agrobacterium tumefacien by Potato Disc Assay method. All of extracts from root sample exhibited antitumor activity against Agrobacterium tumefacien after 5 days and 7 days periods of observation
Collections
Download
Year
- 2018
Author
-
Thandar Aung
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)