Abstract

• Artificial insemination (AI) known as instrumental insemination, is a practice that is popularity among queen breeders. It provides a valuable tool to control the random mating process and is essential for bee breeding and research requiring specific crosses. Three study sites have been chosen as site (I) in Shan State (Aung Ban), site (II) in Bago Region (near Taungoo University) and site (III) in Mandalay Region (Yamethin). Study period lasted from December, 2017 to December, 2018. The study sites were chosen based on the migratory beekeeping method by flowering plants and seasonal changes condition of Myanmar. Artificial Insemination tool was composed of artificial insemination device, microscope, CO2 as anesthesia. Artificial Insemination process have inseminated on 30 virgin queen bees in 2018, totally. In site (I) in Taung Lay Lone, Shan State, AI process have inseminated on ten virgin queen bees, but none is survived and oviposition due to severe weather condition. Artificial insemination process has inseminated on ten virgin queen in site (II), of near Taunggoo University, Bago Region. Result has succeeded six AI mated queens in this area. In site (III) of Yamethin, Mandalay Region, AI process have inseminated on ten virgin queen bees have recorded seven AI mated queens. Comparison on the survival rate of Naturally Mated (NM) queen and Artificially Inseminated (AI) queen bees were carried out. The comparison of the oviposition rate on Artificially Inseminated (AI) queen was also done in three study site. The relationship of oviposition rate and mean temperature, mean relative humidity and mean rainfall is carried out in three study sites. SPSS (Statistical Package for Social Science) version 25 was utilized for the statistical tests. The Nonparametric ANOVA test (Kruskal Wallis) was used.