Abstract

• Isolation of nitrogen fixing rhizobia was made from the root nodules of Cow Pea and Black Gram during July to November 2018. Root nodules were collected from the cultivated field of Anauksu Village, Kyaungkone Township, Ayeyarwady Region, Myanmar. The aim of this research was to isolate nitrogen fixing Rhizobium as starter culture of biofertilizer for peas. Isolation of Rhizobium was made by using Yeast Extract Mannitol agar with Congo red and Bromothymol blue. Identification was based on colony morphology, cell morphology, basic staining reactions and motility. One strain of Rhizobium from Cow Pea and one strain of Bradyrhizobium from Black Gram were isolated. These bacteria could be used as starter culture for the production of rhizobia biofertilizers.