Abstract

• The fungus Stachybotrys sp. were studied, glucose as carbon source 2 %, 3 % and 4 % with yeast extract as nitrogen source 0.6 %, 0.7 % and 0.8 % were used to test on Agrobacterium tumefaciens. To optimize the fermentation medium, it was found that glucose 4 % with yeast extract 0.8 % yield the best result. There are seven different media in which 4 % glucose is combined with two nitrogen sources. There are also four different media in which 4 % glucose is combined with three different nitrogen sources. In the present study, different types of fermentation media were formulated by using 4 % glucose combined with different types of nitrogen sources and found out maximal antibacterial activity against Agrobacterium tumefaciens. In the investigation of ages and sizes of inoculum, 72 hrs ages and 20 % sizes of inoculum were optimized for fermentation. In the fermentation studies, the maximum activity reached at 72 hrs of fermentation for the production of antibacterial compound.