Abstract

• In this research paper, fifteen soil fungi were isolated from four different soil samples of Tu Myaung village in Labutta Township of Ayeyarwady Region. A total of fungi were isolated on Low Carbon Agar (LCA) medium and Potato Glucose Agar (PGA) medium by using feeding and chemical treatment dilution methods. These isolated fungi were tested on seven test organisms by using paper disc diffusion assay method for the screening of antimicrobial activities. In the biological properties of isolated fungi, HY-08 showed highest antibacterial activity of clear zone (27.03 mm) against Bacillus pumilus. Therefore, this fungus (HY-08) was selected for further investigation. This fungus was studied for the age of culture and size of inoculum. According to the result, 60 hrs of ages and 20 % of sizes of inoculum were suitable for the best bioactive metabolite production of selected fungus.