Abstract

• In this study, the synthesis of gold nanoparticles from chloroauric acid (HAuCl4) by using chitosan solution as reducing agent. Gold nanoparticles were prepared by adding different volumes (1 mL, 2 mL, 3 mL, 4 mL) of 0.001M HAuCl4 solution to various concentrations of chitosan solution (1 % and 0.5 % w/v). The existence of nanoparticles in colloidal solution was confirmed by Tyndall effect. The characteristic maximum absorption peak at 520 nm in UV spectrum showed the existence of gold nanoparticles. The antimicrobial activity of prepared gold nanoparticles were carried out. Among them the sample prepared from 1 % w/v chitosan and 4 mL 0.001M HAuCl4 was found to be active agent to all tested microorganisms. The synthesized gold nanoparticles was characterized by XRD, SEM, FT IR and EDXRF. XRD analysis of gold nanoparticles in colloidal solution showed the crystalline nature. From the SEM micrograph, the prepared gold nanoparticles were spherical shape. The relative abundance of gold in the prepared sample was investigated by EDXRF. The prepared gold nanoparticles was applied for the treatment of burn wound healing.