Abstract

• In this study, the endophytic fungi were isolated from the leaves of Muntingia calabura L. in family Muntingiaceae. The plant sources were collected at Yadanabon University Campus, Amarapura Township of Mandalay Region. A total of 7 endophytic fungi were isolated by using surface sterilization method. Morphological characters of endophytic fungi were investigated and were coded MCF-01 to MCF-07. In the study of antibacterial activity of endophytic fungi, six fungi showed the act against test organisms. Among them, the fungus MCF-01 showed the highest activity against on Salmonella typhi. This fungus MCF-01 was isolated from the leaves of M. calabura L.. In the fermentation studies, maximum antibacterial activity of fungus MCF-01 on Salmonella typhi reached at 6 days fermentation with 84 hrs ages (22.25 mm clear zone) and 20% sizes of inoculum (23.49 mm clear zone). Furthermore, distinct characters of selected endophytic fungus MCF-01 was observed with the help of microscope. Endophytic fungus MCF-01 can fight back against typhoid fever disease by S. typhi. in humans. Therefore, fungus MCF-01 was selected for the further investigation such as paper chromatography and extraction of antibacterial metabolite.