Abstract

• Endophytic fungi (6 strains) were isolated from the leaf segments of Vitex trifolia L. collected from Kamayut Township in Yangon region. All the characters of vegetative and reproductive parts of the selected plants were checked with the help of available literatures. The leaves of Vitex trifolia L. were surface sterilized and the isolation of endophytic fungi was done on the sucrose yeast extract medium at 30°C for 3-7 days. All 6 different strains were sub-cultured into separate SY agar slants. The antimicrobial activity of 6 isolated endophytic fungi were investigated against 6 kinds of test organisms by paper disc diffusion method. Six types of endophytic fungi were observed to possess very high antibacterial activity. The selected strains T2, T3, T5 and T6 was extracted and isolation of bioactive compound from each selected broth culture by using TLC, UV and IR.