Abstract

• In this study, the samples of Nypa fruticans (wurmb)Thunb. were collected from Toung-Goke Township in Rakhine State. Eleven entophytic fungi were isolated from different parts (leaves, fruit and inflorescences) of Nypa fruticans (wurmb) Thunb. on five different media. The morphological and microscopically characters of isolated strains were carried out at the Microbiology Laboratory, Department of Botany, Dagon University. The colony appearances of isolated fungal strains were circular, irregular and filamentous. The margins of isolated fungal strains were curled, filamentous, and entire. The elevation of isolated fungal strains was raised, flat and convex. The reverse colors of isolated fungal strains were milky white, pale yellow, white, pale orange, pale green, pale brown and orange. The cultural characters of isolated fungal strains were white, deep black, pale orange, pale green, whitish green, greenish white, creaming white. Eleven isolated fungal strains were identified into possible Aspergillus, Fusarium, Cladosporium, Rhizoctonia, Penicillium and Mucor species. Strains NF-1 and 6 were identified as Aspegillus specie. Strains NF-2,4 and 9 were identified as Fusarium species. Strains NF-3 may be as Mucor species. Strains NF-7 may be Cladosporium species. Strains NF-5 may be Rhizoctonia specie. Strains NF-8 was as Penicillium sp. The antimicrobial activity was tested against eight test organisms by using paper disc diffusion method and fermentation was carried out for 7 to 10 days. The fermented broths of all isolated strains NF-5 and 8 showed good antimicrobial activity on the nine day. Secondary metabolites of eleven active strains were extracted with ethyl acetate and butanol. Their antimicrobial activity was also examined on eight test organisms. The crude extracts of two strains (NF-5 and 8) were showed excellent antimicrobial activity on seven test organisms.