Abstract

• An endophytic fungi Rhizoctonia sp. was isolated from the leaves of Combretum indicum (L.) R. A. DeFillips. In the present study, fermentation (one day old) of age of inoculum was the best for fermentation. In the size of inoculum optimization, fermentation (2%) showed the best antimicrobial activity on six test organisms and fermentation (pH 5) of pH utilization was the best for the production of bioactive compounds according to the results of inhibitory zones of their antimicrobial activity against six test organisms. In the study of extraction of metabolites by using different solvents system, the extracts with methanol, ethanol, ethyl acetate and n-hexane showed the high activity on six test organisms: Bacillus pumilus, Bacillus subtilis, Candida albicans, Micrococcus luteus, Pseudomonas sp. and Staphylococcus aureus. Among them, ethyl acetate extract showed the best antimicrobial activity.