Abstract

• In this experiment, the plantlets were initiated from seeds of Pholidota articulata Lindl. (Kwyetmeee pan myokywe). The experiment was carried out at Plant Tissue Culture Laboratory of the Htone Bo Agricultural Research Farm, Taunggyi, Department of Agricultural Research from October, 2022 to March, 2023. This study was focusing on evaluating the effect of natural additive as plant growth regulators (PGR). The additive used in this study was 100 ml of coconut water (CW) that supplemented in Murashige and Skoog (MS) medium. Four treatments such as T1 (MS only), T2 (half MS), T3 (Full MS and CW) and T4 (half MS and CW). Each treatments of 6 replications and experimental design was set up using Completely Randomized Design (CRD). Murashige and Skoog (MS) and Half MS media were used to study their effects in seed germination and protocorm development. Maximum percentage of seed germination was observed in Half MS and coconut water (T4) medium and minimum percentage of seed germination was in MS only medium (T1). The best protocorms for plantlet development i.e MS media was supplemented with various combinations of Cytokinins (BAP) and Auxin (NAA). Therefore, in vitro developed protocorms were sub-cultured on the Murashige and Skoog (MS) medium, supplemented with coconut water and different concentrations of 6-benzylaminopurine (BAP) and α-naphtalene acetic acid (NAA). The highest number of shoot and number of leaves was observed in MS medium supplemented with 3mg.l-1 BAP.