Abstract

• DNA extraction is one of the most important parts of molecular genetics. In this study, the genomic DNA were extracted from the tender leaves of C. annuum L by using modified Dellaporta method (Sharma et al., 2000). Then, the quality of extracted genomic DNA was amplified by Polymerase Chain Reaction (PCR) technique and checked by gel electrophoresis and NanoDrop spectrophotometer analysis. The result of NanoDrop spectrophotometer revealed that DNA was yielded 336.7 ng/µL concentration with A260/A280 ratio of 1.97 and with A260/A230 ratio of 2.26. That isolated DNA has no protein and others impurities contaminations. The gel electrophoresis gave the intact DNA band and PCR product had the expected band (~500 bp). Moreover, the isolated DNA was purified by five different methods. It was revealed that protocol 1 (PEG 8000) obtained 166.32 ng/µL with 1.78 ratio of A260/A280 that indicate the good quality of DNA from the tender leaves of C. annuum L.