Abstract

• The deccan carp Labeo potail and striped catfish Pangasianodon hypophthalmus were obtained from Arrthit Private Fish Farm, Patheingyi Township to investigate the chromosomal configurations of mitotic cells division by treating with mitotic inhibitor during January to August 2022. The colchicine concentration 0.30 % and 0.50 % were injected into the musculature of both fishes depend on the fish weight ml/g. The various organs such as oral cells, gill filaments, liver, kidney and blood cells of these fishes were treated for durations 3 hrs, 4 hrs 30 mins, 5 hrs and 6 hrs in L. potail. However, 5 hrs, and 5 hrs 30 mins were applied in P. hypophthalmus. The hypotonic solution 0.56 % KCL was used to cell explosion. The highest frequency of interphase stage was observed in blood cells 93.97 % (n=187), kidney 94.21 % (n=114), 91.46 % (n=439), and prophase stage was also found in kidney 38.10 % (n=16) and blood cells 56.19 % (n=168) in both fishes. The most frequent distribution of metaphase stages was observed in the colchicine concentration 0.50 % for duration 4 hrs 30 mins with 0.56 % KCL for duration 1 hr in L. potail whereas 5 hrs, 5 hrs 30 mins in P. hypophthalmus with 0.56 % KCL for duration 1 hr. The most accelerated mitotic stage of cells such as interphase, prophase, metaphase, anaphase and telophase were observed. These results will provide not only the basic information of mitotic technique of cell division but also resolve the mitotic check point of the chromosomal configuration for other freshwater fishes.