Abstract

• The Asian green mussel is widely distributed along the Indo-pacific region, spanning from Japan to New Guinea and from Persian Gulf to South Pacific Islands. The objective of this research is to identify the green mussel species through a comprehensive approach utilizing both morphological and molecular analyses, specifically targeting the mitochondrial cytochrome “c” oxidase subunit I (COI) gene sequences. The relation between shell length (mm), wet tissue weight (g), dry tissue weight (g) and sex of the green mussels were measured. Regarding sexual differentiation, high numbers of male green mussels were abundant than female green mussels. Genomic DNA was extracted using the pet NAD Nucleic Acid Co-Prep Kit, resulting in a sequence length of 660 base pairs (bp). The phylogenetic analysis of the specimens involved a comparison of their sequences with others deposited in GenBank, revealing a close clustering with Perna viridis species, supported by a robust bootstrap value of 100%. This discovery holds significance for taxonomic identification and contributes to the advancement of mariculture development.