Abstract

• The soil microorganisms were isolated from the various soils collected from five different places: Yay Kyaw S-1, Thae Taw S-2, Kaing Yar Gyi S-3, Si Sone Kone S-4, and Kone Thar S-5, Taung Thar Township, Mandalay Region. From the analysis of soil textures, sample No.1 was found to be loamy sand, samples No. 2 and No. 3 were sand, and samples No. 4 and No. 5 were sandy loam. In this study, the soil fungi were isolated by using the feeding method and the physical treatment dilution method. Four fungi were isolated from five different soil samples by the feeding method and six fungi were isolated from five different soil samples by the physical treatment dilution method. The morphology of the isolated fungi were studied in PGA medium. These fungi were used for screening the antimicrobial activities against seven tested microorganisms. The first time, after three days of fermentation, the antimicrobial activity was tested against three microorganisms. Among them, only the isolated fungus from soil sample 1 (Yay Kyaw S-1) showed the highest activity in clear zone (27 mm) on Agrobacterium tumefaciens, followed by (26 mm) on Bacillus subtilis and (25 mm) on Espherichia coli, and no activity against the remaining four tested organisms: Candida albicans, Micrococcus luteus, Pseudomonas fluorescens and Staphylococcus aureus. In the second time fermentation, the isolate fungus from S-1 (32 mm) was especially active against Agrobacterium tumefaciens. This isolated fungus cannot hydrolyze the starch, that is, it did not show the amylase enzyme activity.