Abstract

• The fungus Aspergillus tamarii was isolated from the soil sample of Pone Taung Pone Nyar Area, Magway Region. This fungus showed the antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA). The antibacterial metabolites against MRSA were isolated from the toluene extract of the fermented broth by column chromatography. Sephadex LH-20 resin columns were used finally to purify the metabolites. The active fractions eluted from theSephadex LH-20 column were separately combined and concentrated. These metabolites were checked for purity by thin layer chromatography (TLC), visualized under UV-254 nm and UV-365 nm light. Bioautography on TLC was also carried out to check the retardation factor (Rf) value and purity of the metabolites. From the chromatographic separations, 22.111 mg of metabolite-I and 21.026 mg of metabolite-II were obtained from 0.55 g of the toluene extract of fermented broth. From the physicochemical characterization, UV-vis, and FT IR spectra and comparison with FT IR library, metabolite-I may be a steroid derivative and metabolite-II may be an aromatic derivative compound containing alcohol and carbonyl groups.