Abstract

• The present research deals with the analysis to isolate and elucidate an organic compound and to assess the antiarthritic and anti-inflammatory activities of the aerial parts of Bacopa monnieri that were collected from Twantay Township, Yangon Region. By column and thin layer chromatography separation, the isolated compound structure was elucidated by physicochemical properties and modern spectroscopic techniques such as FT IR, 1H NMR, 13C NMR spectroscopy, and ESI-MS spectrometry, as well as by comparing it with their reported data. In vitro, the anti inflammatory activity of ethanol and watery extracts was evaluated by a nitric oxide inhibition assay. These results did not significantly affect cellular nitric oxide production or cell viability because of cytotoxicity. The ethanol, watery extract, and an isolated compound (bacosine) were assessed for antiarthritic activity. Diclofenac sodium was used as the reference drug. In the egg albumin method, the bacosine compound (IC50 = 105.23 µg/mL) had a more significant antidenaturation effect than the ethanol extract (IC50 = 162.44 µg/mL) and watery extract (172.71 µg/mL). In the bovine serum albumin method, the bacosine compound (IC50 = 118.73 µg/mL) had a more significant antidenaturation effect than ethanol extract (IC50 = 180.58 µg/mL) and watery extract (299.24 µg/mL) compared with diclofenac sodium (IC50 = 73.44 µg/mL), respectively.