Abstract

• The aim of the present study was to isolate and to identify the phytoconstituents from the rhizomes of Globba sherwoodiana (Padein-gno) and to screen their biological activities. The active CHCl3 extract of Padein-gno was separated by silica gel column chromatography which afforded four compounds namely campest-4-en-3-one (A, 4 mg, colourless needle crystal), stigmasta-4,22-dien 3-one (B, 15 mg, colourless needle crystal), stigmast-4-en-3-one (C, 8 mg, colourless needle crystal), and docosyl ferulate (D, 1.0 g, white amorphous powder). All of the isolated compounds, AD were identified by modern spectroscopic techniques including FT IR, 1H NMR, 13C NMR, and HR ESI MS as well as by comparing with the reported data. The antiproliferative activity of the crude extracts of Padein-gno rhizomes and its constituents were screened against four human cancer cell lines [human cervical cancer cell line (HeLa), human lung cancer cell line (A549), human breast cancer cell line (MCF-7), and human gastric cancer cell line (GSU)] and normal human fibroblast cells (WI) by 3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide (MTT) assay. The screening results revealed that the CHCl3 extract showed a higher than the MeOH extract against all the tested cancer cell lines. In addition, the isolated compounds A to D also showed the potent activity with IC50 values ranging from 13.9 to 98.3 µg/mL. In the antibacterial activity, both of the crude extracts exhibited a moderate activity against Gram positive bacteria B. subtilis and S. aureus with MIC values ranging from 25.0 to 100 g/mL. The isolated compounds, A-C were found to show significant antibacterial activity against B. subtilis and S. aureus with MIC values ranging from 3.1 to 25.0 g/mL, respectively.