Abstract

• In the study endophytic fungi, isolated from four species of Fabaceae (Myay-pae, Pe-sin-gon, Pe-Lun and Pe-nauk) collected at Kanpyar Village, Magway Region were undertaken by direct inoculation method. In this investigation, eleven different kinds of fungi TF 1-11 were isolated from collected four species of Fabaceae. TF 1-3 were isolated from Myay-pae, TF 4-6 were isolated from Pe-sin-gon, TF 7-9 were isolated from Pe-Lun, TF 10 and TF-11 were isolated from Pe-nauk. According to the present result indicated that, the surface view and reverse view of endophytic fungi. Five kinds of test organisms (Escherichia coli, Agrobacterium tumefaciens, Staphylococcus aureus, Pseudomonas fluorescens, and Bacillus subtilis) were used in paper disc diffusion method. In the study of antagonistic property of isolated fungi, it was observed that fungus TF-7 exhibited highest activity on Agrobacterium tumefaciens. Therefore, this fungus TF-7 was selected for further investigations. In the fermentation study, it was also found that the optimal ages are 96hrs (24.21 mm) and optimal sizes are 25% (21.91 mm) seed culture at 7 days fermentation. Four fermentation medium (FM 1-4) were used in the fermentation study and it was found that FM-2 showed highest antibacterial activity on the medium. In the preliminary study for the extraction of active compound with four solvents system (Hexane, Chloroform, Dichloromethane and Toluene) were used it was found that chloroform provided highest antibacterial activity on Agrobacterium tumefaciens. The selected isolated fungus TF-7 was identified as Trichoderma hamatum