Abstract

• The present study, eleven fungi were isolated from bean crop soil in Vegetable and Fruit Resources Development Centre, Hlegu Township and twelve fungi were isolated from paddy mill soil in Daik-U Township. Twenty-three fungi were screened by visible plate technique using Potato Dextrose Agar medium (Supplement with 1% Casein). Among all isolated fungi, eight proteolytic fungi were showed clear zone around the fungal colony on Skim Milk Agar (SMA) medium for the preliminary study of proteolytic fungi. Although four proteolytic fungi (V2,V5, D4 and D12) exhibited prominent enzyme activity about (2.5cm for five days, 3.5cm for four days, 2.2cm for four days and 2cm for six days) respectively based on the ratio of diameter of the clearing zone and colony. These four fungi were selected for identification of their colony morphology and spore formation. In the identification of four selected fungi, two Penicillium sp. V2 and V5 were isolated from bean crop soil and one Penicillium sp. D4 and one Cladospordium sp. D12 were isolated from paddy mill soil.