Abstract

• In this study, leaves of a Myanmar indigenous medicinal plant, Eupatorium odoratum L. (Taw-bizat) were chosen for the investigation of phytochemical constituents andsome of the biological activities such as antitumor, antimicrobial, antioxidant and acute toxic properties. The phytochemical constituents, nutritional values and elemental contents were analyzed on the leaves sample by using appropriate reported methods. The methanol extract (25 μg/disc) of the leaves sample was found to possess the antitumor activity against the tumor producing bacteria: Agrobacterium tumefaciens isolated from gall tissues of leaves of Sandoricum koetjape Merr. (Thitto). The crude extracts such as pet-ether, ethanol, methanol and ethyl acetate extracts were observed to be more potent than watery extract in antimicrobial activity against Bacillus subtilis, Staphylococus aureus, Pesudomonas aeruginosa, Bacillus pumilus, Candida albicans and Escherichia coli. However, the watery showed the antioxidant activity (IC50= 13.07 μg /mL) higher than ethanol extract (IC50= 56.14 μg /mL), determined by DPPH radical scavenging assay method. Both of watery and ethanol extracts did not show acute toxic effect up to 5000 mg/kg body weight dose on albino mice. From the separation of silica gel column chromatographic method, a bioflavonoid compound, 2´-hydroxy-4,4´,5´,6´-tetramethoxy chalcone(143-144 .C, 0.12 %) was isolated from the active pet-ether extract of E. odoratum leaves. It was identified by UV-Visible, FT IR, 1HNMR, 13C NMR, and EI-MS spectroscopic methods and also by comparing with the reported data. This isolated chalcone compound was observed to exhibit higher antioxidant activity (IC50= 9.69 μg /mL) than the watery and ethanol extracts.