Abstract

• The research deals with phytochemical and medico-chemical investigation of Auilaria agallocha Roxb. (Thit-mhwae) (A. agallocha) leaves and barks. The samples were collected from Katawe village, Thayatchaung Township, Tanintharyi Region. Antimicrobial activity of three different extracts (PE, EtOAc, EtOH) of A. Agallocha leaves and barks were determined by agar well diffusion method. Test microorganisms were B. subtilis, S. aureus, P. aeruginosa, B. pumilus, C. Albicans and E. coli. EtOAc extracts of leaves and barks of A. Agallocha showed the higher antimicrobial activity than other extracts. The cytotoxicity of Thit-mhwae leaves and barks of EtOH and water extract were tested by brine shrimp cytotoxicity bioassay. According to results, the ethanol extract of leaves showed strong cytotoxic effect at LD50 = 23.56 g/mL whereas other extracts (ethanol bark, watery leaves and bark) showed cytotoxic to brine shrimp at LD50 = 33.34, 58.50, 60.70 g/mL respectively. Therefore, the EtOH extract of leaves was the most potent than other extracts because of the lowest LD50 value and strong cytotoxic. But all of these samples were lower activity than standards K2Cr2O7 (LD50 = 4.38 g/mL) and greater activity than caffeine (LD50 = 1000 g/mL) in cytotoxicity.