Abstract

• Lipolytic fungi were isolated from different sources. Soil sample was collected from the car workshop as fuel oil contaminated soil, Thuwana Township, Yangon Region, Myanmar. Other samples were collected from pork sausage and cheese. Fungal strains were directly isolated from 2 different sources. Diluted soil (concentration - 10-3 , 10-5 , 10-6 , 10-7 , 10-8 , 10-9 ) was used to culture fungi. Fungal strains were cultured on Potato Dextrose Agar (PDA) medium. Lipolytic fungi were screened using Tributyrin Agar (TBA) medium. The isolated fungi were identified by their pure colony morphology and spore formation according to the references. In the present study, three different types of lipolytic fungi were observed from three different sources. Some optimal parameters such as fermentation period, substrate, incubation temperature and initial pH were investigated for lipase activity. Lipase from Aspergillus sp. (1) (isolated from pork sausage source) showed the maximum lipase activity at pH 6.0 and 40ºC. Lipase produced from Penicillium sp. (isolated from cheese source) exhibited the greatest lipase activity at 45 ºC and pH 8.0, while the highest lipase activity from Aspergillus sp. (18) (isolated from fuel oil contaminated soil) was at 50 ºC and pH 8.5.