Abstract

• Endophytic fungal strain Cephalosporium sp. was isolated from the woods of Hesperethusa crenulata (Roxb.) Roem. For extraction, separation, isolation of ten-liter fermentation and antimicrobial activity of the fermented broth on eight test organisms were conducted at Microbiology Lab, Department of Botany, University of Yangon. Antimicrobial activity of the fermented broth indicated highly activity against eight test organisms. After fermentation, the filtrate was extracted on Ambilite XAD 16 resin column with methanol. The methanol extract showed good antimicrobial activity than on Aspergillus flavus, Bacillus subtilis, Candida albicans, Escherichia coli, Malassezia furfur, Micrococcus luteus, Salmonella typhus and Staphylococcus aureus. Separation and isolation of the bioactive compounds from the methanol extract were carried out by using silica 34 gel columns with various solvent systems at Microbiology Lab, Department of Botany, University of Yangon. The seven compounds (A to G) were isolated from the methanol extract of 10 L fermentation. Among them, three terpenoid compounds were mentioned in this paper. Among the isolated compounds, the compound A, B and C were terpenoid compounds and they indicated antibacterial activity on five test organisms. Therefore, these compounds are good to inhibit diarrhea and fever on man as well as crown gall and leaf blight diseases on plants.