Abstract

• Seventeen different kinds of selected mangrove leaves collected at Ma Gyi coastal area, Shwe Taung Yan Township, Ayeyarwady Region during monsoon period, 2016. The selected endophytic fungus, Aspergillus turcosus was isolated from Bruguiera cylindrica (L.) Blume (Byu-Kyet-Tet). In the investigation of antimicrobial activities, this endophytic fungus exhibited highly antibacterial activity on Agrobacterium tumefaciens IFO5431. Based on the growth kinetics of this fungus, it was found that 54 hr of ages and 10% of sizes of inoculum were suitable for the fermentation. The good production of antibacterial metabolite from the endophytic fungus was occurred in glycerol and corn powder as carbon sources and in peptone and beef extract as nitrogen sources against Agrobacterium tumefaciens. Five fermentation medium (FM-1, FM-2, FM-3, FM-4 and FM-5) were prepared for the fermentation study, according to the results of the effects of carbon and nitrogen sources utilization on the fermentation. It was observed that FM-1 medium was the most suitable for the production of antibacterial metabolite.