Abstract

• Codariocalyx motorius (Houtt.) H.Ohashi belonging to family Fabaceae was collected from Pyin Sar village, Pyin Oo Lwin Township during the flowering and fruiting periods from August to November, 2017. The botanical identification, preliminary phytochemical screening, physicochemical evaluation, elemental analysis and antibacterial activities of leaf extracts were carried out. According to preliminary photochemical examinations, the flavonoids, glycosides, phenolic compounds, polyphenols, saponins, amino acids, carbohydrates, reducing sugars and tannins were present while alkaloids, phytosterols and cyanogenic substances were absent. Physicochemical characterization showed that water soluble ash was found more than acid insoluble ash. The studied species showed more amounts of water extractable matter than ethanol, ethyl acetate and pet ether extractable matter. The elemental analysis showed that potassium, calcium, sulphur, iron, manganese, zinc and copper were present. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were detected according to microdilution method. The antibacterial activities were determined with four extracts, namely pet ether, ethyl acetate, ethanol and aqueous against on gram-positive bacteria; Enterococcus faecalis, Staphylococcus aureus and gram-negative bacteria; Eschericha coli, Pseudomonas aeruginosa. The MIC values were between 3.9 mg ml-1 to 125 mg ml-1 and MBC were 31.25 mg ml-1 to >250 mg ml-1 . This plant showed more sensitive effects on gram-positive bacteria than gram-negative bacteria. These results showed that C. motorius (Houtt.) H.Ohashi may be useful to treat diseases caused by Enterococcus faecalis and Staphylococcus aureus infections.