Abstract

• In this study, twenty six fungi were isolated from nine different soil samples for the production of antibiotics. The antimicrobial activities of isolated fungi were tested by ten test organisms. The bioactive metabolites of Penicillium purpurogenum were extracted by using n-BuOH solvent. The antifungal activity of n-BuOH extracts from the fermented broth of P. purpurogenum against C. albicans was examined. Separation of bioactive compounds by fractionations of the crude extract in silica gel column chromatography eluted with co-solvent in gradient was able to separate almost all impurities. From the analysis, four compounds (I, II, III and IV) were obtained. Characterization and classification of four compounds were performed by some chemical reagent tests and some modern spectroscopic techniques such as UV and FTIR. The isolated antifungal compounds could be applied as medicine to treat infectious diseases such as candidiasis, dermatophytosis, meningitis and arthritis which are related to infections of C. albicans.