Abstract

• In the study, soil fungi were isolated from soil samples which were collected from the Pathein University Dhamma Yone campus and then air-dried at room temperature. After three days, ten different soil samples were isolated by feeding method and physical treatment dilution method. Thirty-one soil fungi were isolated from ten different soil samples. Among them isolated soil fungus KM-16 showed the highest antimicrobial activity against Escherichia coli. In this investigation, selected soil fungus (KM-16) was utilized by using paper chromatography for extracting of antibacterial metabolites. The extraction of antibacterial metabolites was carried out by Thin Layer Chromatography and antibacterial metabolites could be isolated from 20 Liters of fermented broth of KM-16.