Abstract

• In this study, fungal strain (YY-13) isolated from soil sample in Minhla Area, Magway Region were intended to produce antibacterial metabolites. In addition, it was focused on the growth and fermentation conditions of isolated soil fungus (YY-13) against Escherichia coli by using agar well diffusion method. Moderate growth and the best antibacterial activity of YY-13 were observed both in carbon and nitrogen sources. The addition of potato powder as a carbon sources resulted better growth and the inhibition zone reached (32.78 mm) in glucose. In the nitrogen sources, the maximum growth of YY-13 was found in peptone and the highest antibacterial activity was obtained by using the sodium nitrate (28.39 mm). The fermentation conditions of YY-13 were studied by the effect of age, size of inoculum, temperature, pH, static and shaking culture. In this investigation, YY-13 was found that 25% of size of inoculums and 84 hrs of age of old culture were suitable conditions. And then, the effects of different temperature and pH range were also determined. According to these studies, temperature 30 ˚C and pH-6 were found to be the best conditions of antibacterial activity against E.coli. In the comparison of static culture and shaking culture of YY13, the static culture exhibited the higher antibacterial activity (26.91 mm) than that of shaking culture (20.38 mm).