Abstract

• The present study is to focus describe and identify the specific bacteria from the nodules of Pisum sativum L. The plant samples were selected from the cultivated field of Kachin State and it carried out to the laboratory of Agricultural Microbiology Section, Agricultural Research Center, Yezin, Nay-pyi-daw. This research was conducted from November 2018 to December 2019. The media YEMA, CRYEMA and BTBYEMA were used as selective media for isolate bacteria. After culturing, colony characters and cell morphological characters of isolate bacteria were studied. Colonies of isolates were sticky appearance, circular, convex (raised), smooth, entire margin and white (translucent). Cell morphological characters were medium size, rod shaped, motility and gram-negative. Moreover, the characterization was done by biochemical tests. As results, the isolated indigenous bacteria were faster grower. The positive chemical reaction were showed Indole, Methyl red, Catalase, Urease hydrolysis, starch hydrolysis, Glucose peptone agar, Glucose, Sucrose and Mannitol tests. The negative chemical reactions were showed in lactose fermentation, Citrate utilization, Gelatin hydrolysis, Methyl blue and Mannitol salt agar experiments. According to the character results, isolated indigenous bacteria from nodules of Pisum sativum L. was confirmed as genus Rhizobium.