Abstract

• Endophytic fungi is a rich source of novel organic compounds with interesting biological activities and a high level of structural diversity. In the present study, endophytic fungus MHA-10 was isolated from the leaves of Dioscorea birmanica Prain and Burkill. The pure fungal culture was inoculated in fermentation medium with different growth parameters. In the present investigation, the effect of pH was studied by varying pH 3, 4, 5, 6, 7, 8 and 9. The maximum antibacterial activity was recorded at pH 7 (21.86 mm, inhibitory zone) against Bacillus subtilis. Antibacterial activity was observed at different temperatures such as 20ºC, 25ºC, 30ºC, 35ºC and 40ºC. Maximum antibacterial activity by fungal isolate MHA-10 was recorded at 30ºC (19.84 mm, inhibitory zone) against Bacillus subtilis. In the comparison of static and shaking culture, the antibacterial activity of shaking culture (20.89 mm, inhibitory zone) was more than that of static culture (19.01 mm, inhibitory zone). Maximum antibacterial activity reached at 5 days fermentation period (22.38 mm, inhibitory zone) against Bacillus subtilis. In the paper chromatography study, ethyl acetate is suitable for the extraction of crude extract from the fermented broth. Based on the macroscopical and microscopical characters, fungus MHA-10 was identified as Trichoderma sp.