Abstract

• Endophytes are ubiquitous organisms that live within the host plants without causing any apparent symptoms of disease. In the present study the endophytic microorganisms were isolated from plants Garcinia mangostana L. and 12 endophytic bacteria were successfully screened from different parts of leaves, bark and fruit. Characterization of endophytic bacteria was performed based on the morphology, biochemical characteristic and the antimicrobial properties. Screening of potential antibacterial metabolites was done by using paper disc diffusion method and bioautography. The antibacterial activity was tested against eight test organisms. Cell morphology and colony characters of isolated bacteria were investigated and three bacteria which show antimicrobial activity (B-2, B-7 and B-9) were selected and applied in the fermentation was media using nutrient broth for two days. After separate fermentation, the crude compounds were extracted by using ethyl acetate and butanol and partially purified by TLC. The results of TLC showed the presence of Quercetine, Tannin and Xanthone in each extract of B-2, B-7 and B-9.