Abstract

• The present paper was studied by the fermentation optimization and selection of solvent to extract antibacterial compound from isolated soil fungus NLF-12. Twenty fungi were isolated from four different soil samples of Beikthano Ancient City, Taungdwingyi Township, Magway Region. Antimicrobial activity of all fungi was tested by agar well method on four test organisms and nine fungal strains showed the activity. Among them, soil fungus NLF-12 showed the highest antibacterial activity (30.54 mm) on Micrococcus luteus. Therefore, it was selected and the fermentation optimization of fungus NLF-12 were carried out by proper age, size, different carbon and nitrogen sources, fermentation medium (FM), pH, temperature, static and shaking culture. In the results of paper chromatography, ethyl acetate was the most suitable solvent for extraction of antibacterial secondary metabolites from fermented broth of fungus NLF-12 and Rf value had 0.92 on Micrococcus luteus. These results indicated that the selected soil fungus may be utilized by the optimal fermentation conditions for the screening of antibacterial activity.