Abstract

• Extraction of antifungal metabolites from selected strain Actinomycetes was carried out by using paper chromatography and four solvent systems. Ethyl acetate was the most suitable solvent and Rf value had 0.86. Crude ethyl acetate extract (2.0 g) was obtained from 10 L of fermented broth and purified over silica gel column chromatography. The ethyl acetate extract was carried out by employing various solvent systems on Thin Layer Chromatography (TLC). The results showed well separated spots on TLC by using the solvent system (pet ether: ethyl acetate). By silica gel column chromatographic separation, compound A (23 mg, white amorphous form) was isolated and subjected to various examinations such as chemical reagent tests, ultraviolet (UV) and FTIR (Fourier Transform Infrared). Based on the results of physicochemical properties and spectroscopic methods, compound A may be flavonoid. In the investigation of minimum inhibitory concentrations (MICs), the MIC value of compound A was 2.5 µg/mL on Candida albicans.