Abstract

• The objective of the present study is to investigate bioactive metabolites from Dendrobium pulchellum roots. Bibenzyl derivatives extracted by acetate ethyl namely, 4-(4-hydroxy3-methoxyphenethyl)-2, 6-dimethoxyphenol (1) and 4-(3,4-dimethoxy-phenethyl)-2,6-dimethoxy phenol (2) were isolated by using separation techniques such as thin layer and column chromatography. The structure elucidation the isolated compounds was performed based on NMR and mass. Moreover, the antioxidant activity of crude extract was evaluated by using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Furthermore, the acute toxicity and antimicrobial activities of crude extracts were also examined.