Abstract

• The rhizome of L. (Lin-ne) used in the treatment of diarrhoea and dysentery in traditional Myanmar medicinal system was chosen for present study. The aim of the study is to isolate asarone from the rhizome of L. (Lin-ne) and antibacterial screening of its crude extracts and asarone. At first, four crude extracts of this plant were prepared by using various solvents; petroleum ether, ethyl acetate, 96 % ethanol and 50 % ethanol. antibacterial activity of four crude extracts was investigated against 19 bacterial strains by using agar disc diffusion method. Among the four crude extracts, the most active ethyl acetate extract was selected for isolation of active compound by using column chromatographic method. The isolated compound, asarone (0.712 %) was identified by TLC and spectroscopic methods; UV, FT IR, GC-MS and then tested on 11 bacteria; species, A, species, ATCC,   YCH 149,  species, and O1 by agar disc diffusion method. In addition minimum inhibitory concentration (MIC) of asarone was determined by microtitre plate dilution method and the MIC value 0.06 mg/mL of asarone on tested bacteria,  LT and  was found.