Abstract

• A total of 15 fungi were isolated from four different soil samples collected from Pathein Area. All 15 fungi showed no antimicrobial activity against eight test organisms except Agrobacterium tumefaciens. Among them, fungus TH-15 exhibited more activities than other fungi. After examining the biological activities, TH-15 was found to be similar with those of genus Stachybotrys sp. In the fermentation studies, the maximum activity reached at 72 hrs of fermentation for the production of antibacterial compound. After fermentation with optimal parameter, the broth was adjusted pH 7.0 and studied by paper chromatography. Thin layer chromatography (TLC) by using the crude extract to obtain the basal data. The purification of antibacterial compound was undertaken by silica gel column chromatographies with various elution solvents collected by Preparative Thin Layer Chromatography (PTLC).