Abstract

• In this research work,the dried Kan-zaw seeds were collected from Myoe Haung Village, Palaw Township, Myeik District, Tanintharyi Region. Firstly the phytochemical investigation of dried Kan-zaw seed was carried out. Secondary plant metabolites such as flavonoids, tannins, carbohydrates, saponins, phenols, glycosides, reducing sugars, terpenoids, steroids and alphaamino acids were found to be present in Kan-zaw seed. The oil was extracted from dried Kan-zaw seed by using oil press machine. The physico-chemical characteristics of expressed Kan-zaw seed oil such as acid value, peroxide value, iodine value and free fatty acids were studied. FTIR analysis of functional groups present in the Kan-zaw seed oil was also conducted. Fatty acid profile of expressed Kan-zaw seed oil was evaluated by Gas Chromatography Flame Ionization Detection (GCFID) method. Based on the expressed Kan-zaw seed oil, pain relief balm was formulated using D-optimal mixture design of Design Expert Software trial version 11.0. Optimum formulation of pain relief balm was judged by its properties such as pH, viscosity and spreadability. Pain relief balm with pH 6.52, viscosity 11613.33 cP and spreadability 29.56 gcm/s was found as optimum composition. Skin irritation test for prepared pain relief balm was conducted in rabbit model and there were no skin rashes, inflammation and itching or redness on applied portions for 96 hours. Consumer acceptance for pain relief balm with optimum composition was studied by 9 point-hedonic scale. Total score for pain relief balm was 6.7 and it was between like slightly and like moderately.