Abstract

• The purpose of this study was to determine the biomedical constituents and antimicrobial properties of the crude extracts isolated from the leaves of E. odoratum collected in 2017 at Yadanabon University campus, Mandalay, Myanmar. The crude extracts, isolated by using maceration method with ethanol and hexane as solvents, were analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The result showed the presence of n-Tetradecanol (52.98%, 37.42%) in both ethanol and hexane extracts but methyl hexadecanoate (40.47%) was recorded only in hexane extract as the main constituent. 2E-dodecanol (55.25%) was found to be the dominant constituents in ethanol extract of the leaves. The antimicrobial activity was evaluated by the paper disc diffusion method. Antimicrobial tests showed that ethanol extract of the leaves was active against all the clinically isolated gram-positive and gram-negative bacteria tested. The hexane extract was active against E. coli and V. cholerae but inactive against K. pneumoniae and all tested gram positive bacteria. The ethanol extracts which demonstrated good antibacteria activities and also showed better minimum inhibitory concentration (MIC) values on V. cholera and S. aureus (31.25 µg/ml). The results suggest once again that the antimicrobial activity of the extracts of the leaves is a resultant of the antibacterial properties of the major and minor components in their chemical composition. The skin irritant property of prepared ointments were examined by albino rabbit and there were not shown skin irritant property.