MOLECULAR FORMULA DETERMINATION AND STRUCTURE ELUCIDATION OF BIOACTIVE PORPHYRIN DERIVATIVE COMPOUND ISOLATED FROM THE WHOLE PLANT OF Corallodiscs langinoss (WALL. ex R.Br.) BURTT
Abstract
- In this research paper, Corallodiscus lanuginosus (Wall. ex R.Br.) Burtt which is one of the Myanmar indigenous medicinal plants known as Pan ma o was selected for chemical analysis. The preliminary phytochemical screening of the whole plant of Pan ma o was carried out, which indicated the presence of alkaloid, steroid, terpenoid, glycoside, lipophilic, polyphenol, saponin, phenolic and tannin, respectively. Antimicrobial activities of the crude extract of the whole plant of Pan ma o were tested in various solvents system by using agar well diffusion method on six selected organisms. The ethyl acetate crude extract of Pan ma o gives rise to high activities on all selected organisms such as Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and E.coli. Moreover, porphyrin derivative compound was isolated from the whole plant of Pan ma o as indigo crystal (19.6 mg) by thin layer and column chromatography separation methods. The yield percent was found to be (0.529%) based upon the EtOAc crude extract. Moreover, antimicrobial activities of this compound were rechecked by using agar well diffusion method. The ethyl acetate extract of this compound responds high activities on all selected organisms except Staphylococcus aureus. In addition, the molecular formula of this compound could be determined as C30H35N9O4 (Hydrogen Deficiency Index = 18), applying some spectroscopic methods such as FT IR, 1H NMR (500 MHz), 13C NMR (125 MHz), DEPT and FAB-mass spectral data respectively. The complete structure of this porphyrin derivative compound could be elucidated by DF-COSY, HMC, HMBC and DEPT spectroscopic methods. The elucidated compound could be described as below.
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Year
- 2018
Author
-
Thinn Myat Nwe
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)