Abstract

• The fabrication of silver nanoparticles by using watery extracts of Eupatorium odoratum L. (Taw-Bizat) and Cymbopogon citratus Stapf (Lemon Grass) leaves was mainly conducted. Silver nitrate was used as a metal precursor and two selected leaves extracts were applied as biomimetic routes of reducing agent for synthesis of silver nanoparticles (AgNPs). The surface morphology of fabricated silver nanoparticles (AgNPs) were performed with FESM (Field Emission Scanning Electron Microscopy). From the result of XRD (X-ray diffraction), the average particle size of TBAgNPs (32 nm) from E. odoratum and LGAgNPs (38 nm) from C. citratus watery extracts were observed. The size distribution of each prepared (AgNPs) was analysed by using advanced techniques Zeta potential-DLS (Dynamic Light Scattering). The localized surface plasmon resonance bands for formation of TBAgNPs (440 nm) and LGAgNPs (435 nm) in the ratio of 1:3 and 1:5 were exhibited in UV-visible spectra. The role of selected stabilizing agent of E. odoratum and C. citratus leaves extracts and the effect of stirring time on synthesis of AgNPs was reported. Furthermore, the laser beam of reflected rays as observed in the fabrication of AgNPs by the Tyndall effect. In addition, the antioxidant activities of TBAgNPs and LGAgNPs (49.27 g/mL, 94.32 g/mL) were determined by DPPH radical scavenging assay method. The antimicrobial activities of two kinds of leaves extracts and the prepared TBAgNPs and LGAgNPS were discussed against different strains of microorganisms.