Abstract

• This research work deals with investigation of some bioactive phytoconstituents of Morus alba L. (Po-sa) plant collected from Pyin-Oo Lwin Township, Mandalay Region. By silica gel column chromatographic separation technique, three compounds were isolated from selected plant. Umbelliferone (1,0.0045%) Scopoletin (2, 0.0009 %)m.pt 201ºC ) and protocatechuic acid (3,0.0036 %) from ethyl acetate extract of Po-sa leaves. The isolated compounds were identified by physic-chemical properties and modern spectroscopic techniques such as UV, FT IR, 1H NMR, 13C NMR and EI MS spectrometry as well as by comparing with their reported data. Antioxidant activity of Po-sa (leaves) was also investigated by using DPPH (1, 1'-diphenyl-2-picrylhydrazyl) radical scavenging assay. The IC50 values of EtOAc, EtOH and watery extracts of leaves were 2.39 µg/mL, 2.11 µg/mL and 2.15 µg/ml respectively. The EtOH and watery extracts of leaves showed more radical scavenging activity than EtOAc extract. In addition, Umbelliferone, scopoletin and protocatechuic acid isolated from Po-sa (leaves) also showed radical scavenging activity determined by using semi quantitative DPPH staining method.