ISOLATION OF PIGMENTED FUNGUS FROM LOBOPHORA VARIEGATA (J.V.LAMOUROUX) AND EXTRACTION OF ANTIBACTERIAL ACTIVITY AGAINST ON ESCHERICHIA COLI AHU5436
Abstract
- In this investigation, the isolation of pigmented fungus has been conducted from brown seaweed. The samples of brown seaweed species, Lobophora variegata (J.V.Lamouroux) was collected the natural beds from the rocky shore of Minn- Land coastal area, Ngwe- Saung region, Pathein township. The isolation of pigmented fungus was carried out by Washing method. During the preliminary study of antibacterial activity on Escherichia coli AHU5436, the paper disc diffusion assay method was used to check the activity of the fermented broth. The filter paper and four solvents such as 20% NH4CL, water saturated n-butanol, n-butanol- acetic- water (3:1:1) and water saturated ethyl acetate were used for Paper chromatography according the method of Tomita,1988. Antibacterial metabolite was extracted with ethyl acetate from the fermented broth based on the results of (R f value) paper chromatography.
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Year
- 2020
Author
-
Khin Thandar Lin
Subject
- Botany and Marine
Publisher
- Myanmar Academy of Arts and Science (MAAS)