ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM ROOT NODULES OF ARACHIS HYPOGAEA L. (GROUNDNUT)
Abstract
- The present study was to isolate the beneficial nitrogen fixing bacteria Rhizobium from root nodules of Arachis hypogaea L. (Groundnut). Plant sample and rood nodules sample were collected from cultivated field of Munkhrain Quarter, Myitkyina Township in Kachin State. This study was done at Research Center, University of Myitkyina from January to May 2019. The five selected Rhizobium strains ( MTZA 1, MTZA 2 , MTZA 3, MTZA 4, MTZA 5) were subjected to culture on Yeast Mannitol agar ( YMA ) and Yeast Mannitol Broth (YMB). The method of Vincent (1970) was used for isolation of Rhizobium from root nodules. Rhizobium strains were medium sized, rod shaped, Gram negative and no spore. Colonies were sticky appearance, circular, varying from flat to raise shaped, margin entire and milky white colour. The Rhizobium strains were aerobic, motile, turbidity and the positive chemical reaction was observed in Catalase, Lactase, Glucose Peptone agar (GPA), Triple Sugar Iron Agar and Antibiotic test. The negative chemical reaction was observed in Gelatin Hydrolysis and Methylene blue test.
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Year
- 2020
Author
-
MayThazin Aung
Subject
- Botany and Marine
Publisher
- Myanmar Academy of Arts and Science (MAAS)