ISOLATION OF SOIL FUNGI FROM MUDON TOWNSHIP, MON STATE AND THEIR ANTIMICROBIAL ACTIVITY
Abstract
- In this research work, soil samples were collected from five different places of Mudon Township including Kwaiwan, Kawkhite, Tarpaton, Tharyargone and Kyauktalone village, during July 2018. Soil fungi were isolated by the serial dilution method from these samples and cultured on Blaskeslee’s Malt Extract Agar (BMEA Medium), Czapek- Dox Agar (CZA Medium), Malt Extract Agar (MEA Medium), Dichloram Rose Bengal – Chloramphenicol Agar (DRBC Medium), Glucose Ammonium Nitrate Agar (GAN Medium), Potato Glucose Agar (PGA Medium) and incubated for 3-7 days at room temperature. A total of 41 fungal strains were isolated and the surface color of all strains were white, brown , greenish brown, and their reverse color were cream, black, greenish brown, yellowish brown respectively. In the colony morphology, the isolated fungi were small, medium and large in size. The margins of isolated fungi were entire convex, raised, and the form of isolated fungi circular and irregular. Moreover, physicochemical properties of soil from different locations of Mudon Township were analyzed. All fungal strains were tested by eight test organisms for preliminary study of antimicrobial activity. Among them, six strains showed different level of antimicrobial activity. MP- 7 exhibited the highest antibacterial activity (25.05 mm) against and MP- 25 also showed the moderate activity (23.50 mm) on Bacillus pumilus at 5 days. MP- 6 gave the strong antibacterial activity (20.03 mm) against Bacillus Subtilus at 6 days. Especially, MP- 41 showed the moderated antimicrobial activity against all test organisms.
Collections
Download
Year
- 2020
Author
-
Myat Myat Phyo
Subject
- Botany and Marine
Publisher
- Myanmar Academy of Arts and Science (MAAS)