ISOLATION AND ANTIMICROBIAL ACTIVITY OF ACTINOMYCETES FROM CHAUNG-THA AREA AND BIOCHEMICAL CHARACTERIZATION OF SELECTED STREPTOMYCES (TR-2)
Abstract
- A total of 13 actinomycetes were isolated from two samples sea-water and sludge, ChaungTha area, Ayeyarwady region. Among them, 8 strains were obtained from sludge sample and 5 strains from sea water. In the elevation, the isolated strains were circular, raised, convex, umbonate and flat. In the margin and textures, all strains were entire and rough. The colony size of isolated strains were large, small and moderate. The aerial mass colour of all strains was greenish blue, white and centre greenish blue, white in periphery. Their substrate colour was yellow. The antimicrobial activity of all strains were screened by agar well diffusion method on ten test organisms. Of these 13 strains, eight strains showed the antimicrobial activity. Among them, TR-2 showed the highest antifungal activity on Candida albicans. Therefore, TR-2 was selected for biochemical characterization. Positive results were found in methyl red test, citrate utilization test, casein test, mannitol salt broth test, potato plug test, nitrate reduction, catalase and urease test. But, Voges proskauer test, hydrogen sulfide test, gelatin hydrolysis test, esterase activity test, oxidase test and motility test were negative. According to the results, TR-2 was classified as the possible genus Streptomyces sp. Keywords: Sea water, sludge, streptomyces, antimicrobial activity
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Year
- 2020
Author
-
Ma Hnin Thiri Lwin
Subject
- Botany and Marine
Publisher
- Myanmar Academy of Arts and Science (MAAS)