CULTURAL CHARACTERISTICS AND ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC FUNGI FROM LEAVES OF TWELVE MEDICINAL PLANTS
Abstract
- A total of 18 endophytic fungi were screened and isolated into pure culture from the leaves of 12 medicinal plants collected from Pathein University and Mawlamyine University Campus and Taung wine area, Mawlamyine. The isolates were designated as MHA-1 to MHA-18. Among them MHA-10 which was isolated from the leaves of Dioscorea birmanica Prain and Burkill. (Khat-Cho) showed the highest antibacterial activity (18.90 mm clear zone) against Bacillus subtilis. Thus MHA-10 was selected for further study of optimal fermentation parameter to produce antibacterial metabolites. It was recorded that 84 hrs ages of inoculums, 30% size of inoculums were found to provide the best antibacterial activity in fermentation medium-1. Maximum antibacterial activity (18.03 mm clear zone) was found when fermentation media was supplemented with glucose. Among the wide variety of nitrogen sources tested, peptone proved to be the most suitable for antibacterial activity (18.95 mm clear zone) of selected fungus MHA-10 against Bacillus subtilis. FM-1 gave maximum antibacterial activity (20.64 mm clear zone) against Bacillus subtilis.
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Year
- 2020
Author
-
Myo Htaik Aung
Subject
- Botany and Marine
Publisher
- Myanmar Academy of Arts and Science (MAAS)