ISOLATION AND MORPHOLOGICAL CHARACTERIZATION OF FUNGI FROM BEIKTHANO ANCIENT CITY AND THEIR ANTIMICROBIAL ACTIVITY
Abstract
- Soil samples were collected from four different places of Beikthano Ancient city, Taungdwingyi Township, Magway Region. Twenty fungi were isolated from these four different soil samples. Isolation of fungi were undertaken by the chemical treatment method. The soil fungi were isolated by using Low Carbon Agar medium and Malt Extract Agar medium for first culture and Potato Glucose Agar medium for pure culture. The isolated fungi were given as NLF-1 to NLF-20. The surface color of isolated fungi NLF-1 to NLF-20 were white, green, orange white, greenish yellow, yellow, pale brown, black, gray and their reverse color were pale yellow, white, cream, greenish yellow, brown, gray and pink. Antimicrobial activity of isolated fungal strains was evaluated by the agar well diffusion assay with four test organisms. These isolated fugal strains were tested by using 3 days to 10 days old culture. Nine strains showed the antimicrobial activity against Agrobacterium tumefaciens, Aspergillus paraciticus, Micrococcus luteus and Pseudomonas fluorescens. Among them, NLF-14 showed the highest antibacterial activity (32.53 mm) on A. tumefaciens followed by NLF-7 (29.40 mm) and NLF-16 (29.96 mm) respectively. And then, NLF-14 also showed the highest antifungal activity (32.43 mm) on A. paraciticus followed by NLF-9 (31.09 mm) and NLF-6 (30.73 mm). Among all the selected fungi, NLF-12 showed the best inhibition zone (34.85 mm) against M. luteus.NLF-6 showed the highest antibacterial activity (30.26 mm) on P. fluorescens.
Collections
Download
Year
- 2020
Author
-
Khin Nilar Oo
Subject
- Botany and Marine
Publisher
- Myanmar Academy of Arts and Science (MAAS)